Clark, John, Ph.D.
Professor

clarkji@u.washington.edu
G-525
(206) 685-0950

Dr. Clark's COS page --  a complete description of research and for an updated bibliography with abstracts

Lens cells offer a unique opportunity to study the chemical and  molecular basis for the regulation of cell differentiation.  Lens  cells begin opaque and differentiate into transparent cells and the  optical  quality of the lens is the result of a highly synchronized,  multistep process of cell proliferation , migration, and  differentiation.  The transparency and refractile properties of the  lens are determined  by expression of high concentrations of the  alpha and beta/gamma crystallin families of proteins.  The diversity of the  individual crystallins contributes to the complex structural  organization of the  proteins in the homogeneous transparent  cytoplasm.  The structural and functional interactions between  crystallin proteins that are responsible for the development and  maintenance of lens cell  transparency and refraction are the primary  considerations of our research effort.  Quantitative techniques of  protein biochemistry, molecular biology, LASER light scattering spectroscopy, and light and  electron microscopy are used in these studies.  We  are developing new methods for analysis of electron and light  micrographs of cell and tissue structure using 2-D Fourier methods,  fractal analysis and  chaos theory.   
The principles of lens cell differentiation apply to differentiation  of other cells and tissues including red blood cells, skin cells, and  muscle fibers.

Publications
(* indicates student or postdoctoral co-author)

[2010, 2009, 2008, 2007, 2006, 2005, 2004, 2003, 2002, 2001, 2000, 1999, 1998, 1997, 1996, 1995, 1994, 1993, 1992, 1991, 1990, 1989, 1988, 1987, 1986, 1985, 1984, 1983, 1982, 1981, 1980, 1979, 1978, 1976, 1975]

1. TMS Greiling*, M Aose, JI Clark (2010) Cell Fate and Differentiation of the Developing Lens. Inv Ophthalmol. & Vis Sci.  51:1540-1548. PMC Journal - In Process. (PDF)

2. JA Moncaster*, R Pineda, RD Moir, S Lu, MA Burton, JG Ghosh, M Ericsson, SJ Soscia, A Mocofanescu, RD Folkerth, RM Robb, JM Kuszak, JI Clark, RE Tanzi, DG Hunter, LE Goldstein (2010) Alzheimer's disease amyloid-beta lins lens & brain pathology in Down's syndrome PLoS ONE 5(5):e10659. (PDF)

3.  SA  Houck, JI.Clark (2010) Dynamic subunit exchange and the regulation of microtubule assembly by the stress protein human αB crystallin  PLoS ONE 5(7):e11795. (PDF)

2. TMS Greiling* SA Houck* & JI Clark (2009) The zebrafish lens proteome during development and aging. Mol Vis 15:2313-2325. PMCID: PMC2779061. (PDF)

3. TMS Greiling*,JI Clark (2009) Early lens development in the zebrafish: a 3-dimensional time-lapse analysis. Dev Dyn 238:2254-2266. PMC Journal - In Process.

4. TMS Greiling*,B Stone,JI Clark(2009) Absence of SPARC leads to impaired lens circulation. Exp Eye Res .89:416-425. PMCID: PMC2727862. (PDF)

5. S Zigler, PF Kador, JI Clark & M Datiles, (2009)  Medical Treatment of Cataract.in Duane's Ophthalmology , W. Tasman & EA Jaeger, eds. Vol 1, Chapt 75.

6. J Yuen*, Y Li*, LG Shapiro, JI Clark, E Arnett, EH Sage & J Brinkley (2008) Automated, Computerized, Feature-Based Phentoype Analysis of Slit Lamp Images of the Mouse Lens. Exp Eye Res 86:562-57. PMCID: PMC2674242. (PDF)

7. PJ. Muchowski, R Ramsden, QV Nguyen, EE. Arnett,T Greiling*, SK. Anderson & JI. Clark (2008) Noninvasive Measurement of Protein Aggregation by Mutant Huntingtin Fragments or α-Synuclein in the Lens. J Biol Chem 283:6330-6336 PMCID: PMC2650484. (PDF)

8. JG Ghosh*, SA Houck* & JI Clark (2008) Interactive sequences in the molecular chaperone, human αB crystallin modulate the fibrillation of amyloidogenic proteins. Int J of Biochem & Cell Biol 40:954-966. PMCID: PMC2688400. (PDF)

9. TMS Greiling* & JI Clark (2008) The transparent lens & cornea in the mouse & zebra fish eye.  Seminars in Cell & Developmental Biology 19:94-99. PMCID: PMC2674238. (PDF)

10. JG Ghosh*, AK Shenoy Jr*.& JI Clark (2007) Interactions between important regulatory proteins and human αB crystallin. Biochemistry. 46:6308-6317. PMC Journal - In Process. (PDF)

11. ZY Park, R Sadygov, JM Clark, JI Clark & JR Yates III (2007) Assigning in vivo carbamylation & acetylation in human lens proteins using tandem mass spectrometry & database searching. Intl J Mass Spectrometry 259:161-173.

12. JG Ghosh*, SA Houck* & JI Clark (2007) Interactive sequences in the stress protein and molecular chaperone human αB crystallin recognize and modulate the assembly of filaments. Int J of Biochem & Cell Biol 39:1804-1815. PMCID: PMC2743261. (PDF)

13. N Gotoh*, NR Perdue, H Matsushima, EH Sage,Q Yan, & JI Clark (2007) An in vitro model of posterior capsular opacity: SPARC and TGF-β2 minimize epithelial-to-mesenchymal transition in lens epithelium. Invest Ophthalmol Vis Sci 48:4679-87. PMC Journal - In Process. (PDF)

14. JG Ghosh*, SA Houck*& JI Clark (2007) Interactive domains in the molecular chaperone, human αB crystallin modulate microtubule assembly and disassembly. PloS ONE 2:e498, PMCID: PMC1876262. (PDF)

15. JG Ghosh*, SA Houck*,CE Doneanu & JI Clark (2006) The β4-β8 groove is an ATP-interactive site in the α crystallin core domain of the small heat shock protein, human αB crystallin. J. Mol. Biol. 364:364-375. (PDF)

16. L Liu* JG Ghosh* JI Clark & S Jiang (2006) Studies of αB crystallin dynamics by surface plasmon resonance (SPR).  Anal Biochem. 350(2):186-95. (PDF)

17. JG Ghosh*, AK Shenoy Jr*, & JI Clark (2006) N- and C-terminal motifs in human αB-crystallin play an important role in the recognition, selection, and solubilization of substrates. Biochemistry 45:13847-13854. (PDF)

18. JG Ghosh*, MR Estrada* & JI Clark (2006) Structure based analysis of the β8 interactive sequence of human αB crystallin.  Biochemistry 45:9878-9888. (PDF)

19. RO Emerson*, EH Sage, JG Ghosh*, & JI Clark (2006) Chaperone-like activity revealed in the matricellular protein, SPARC.  J. Cell Biochem 98:701-705. (PDF)

20. JG Ghosh*, MR Estrada*, SA Houck* & JI Clark (2006) The function of the beta3 interactive domain in the sHSP & molecular chaperone, human alphaB crystallin. Cell Stress and Chaperones 11:187-197. PMCID: PMC1484519. (PDF)

2005

21. H Matsushima, K Mukai, N Gotoh*, S Yoshida, T Yoshida, M Sawano, T Senoo, Y Obara & JI Clark (2005 ) The effects of drug delivery via hydrophilic acrylic (hydrogel) intraocular lens systems on the epithelial cells in culture. Ophthalmic Surg Lasers Imaging. 36:386-392.

22. JG Ghosh*, MR Estrada* & JIClark (2005) Interactive domains for chaperone activity in the sHSP, human αB crystallin.  Biochemistry 44:14854-69. (PDF)

23. JG Ghosh* & JI Clark (2005) Insights into the domains required for dimerization and assembly of human αB crystallin.  Protein Science 14:684-696. PMCID: PMC227928. (PDF)

2004

24. V Zderic*, J.I. Clark & S.Vaezy (2004) Drug delivery into the eye with the use of ultrasound. J. of Ultrasound in Medicine 23:1349-59. (PDF)

25. C Fong*, C Rosse, JI Clark, LG Shapiro & JF Brinkley (2004) Ontology-Based Image Repository for a Biomedical Research. Proceedings of Med Info. 37:1598-1604.

26. V Zderic*, JI Clark, RW Martin & S Vaezy (2004) Ultrasound & transcorneal drug delivery. Cornea 23:803-811. (PDF)

27. JI Clark (2004) Order and disorder in the transparent media of the eye. Exptl. Eye Res. 78:427-432. (PDF)

28. A Alizadeh*, JIClark, TMSeeberger*, J Hess, T Blankenship & PG Fitzgerald (2004) Characterization of a mutation in the lens specific CP49 in the 129 strain of mouse. Invest.Ophthalmol. & Vis.Sci. 45:884-891. (PDF)

29. H Matsushima, K Mukai, Y Obara, S Yoshida & JI Clark (2004) Analysis of cytoskeletal proteins in posterior capsule opacification after implantation of acrylic & hydrogel IOLs. J. Cataract Refract. Surg. 30:187-194. (PDF)

30. TM Seeberger*, Y Matsumoto, A Alizadeh*, P G Fitzgerald & JIClark (2004) Digital image capture and quantification of subtle lens opacities in rodents. J. of Biomed Optics. 9: 116-120. (PDF)

2003

31. RR Ansari, J F King, T Seeberger* & JI Clark (2003) Early detection of cataract and response to pantethine therapy with non-invasive static and dynamic light scattering.  Proc. SPIE 4951:168-76; Ophthalmic Technology XIII.

32. A Alizadeh*, JI Clark, TM Seeberger*, J Hess, T Blankenship & PG Fitzgerald (2003) Targeted deletion of the lens fiber cell specific intermediate filament protein filensin. Invest.Ophthalmol. & Vis.Sci.  44:5252-5258.  (PDF)

33. Q Yan, D Blake*, JI Clark  & EH Sage (2003) Expression of the matricellular protein SPARC in murine lens:  SPARC is necessary for structural integrity of  capsular basement membrane.  J. Histochem. Cytochem. 51:503-511. (PDF) 

2002

34. A Alizadeh*, JI Clark, T Seeberger*, J Hess, T Blankenship, A. Spicer and P.G. Fitzgerald (2002 )Targeted genomic deletion of the lens specific intermediate filament protein CP49. Invest. Ophthalmol. Vis. Sci. 43:3722-3727. (PDF)

35. A Avakian, RE Kalina, EH Sage, AH Rambhia*, KE Elliott*, EL Chuang, JI Clark, JN Hwang, P Parsons -Wingerter. (2002) Region-based morphometry of the retinal vascular tree in human eyes with non -proliferative diabetic retinopathy.  Curr Eye Res 24:274-280.

36. V Zderic*, S Vaezy, RW Martin and JI Clark (2002) Ocular drug delivery using 20 kHz ultrasound. Ultrasound in Med. & Biol. 28: 823-829. (PDF)

37. MJ MacCoss, EW Hayes-Macdonald, A Saraf, ER Sadygovl, JM Clark, JJ Tasto, KL Gould, D Wolters, M Washburn, A Weiss, JI Clark, and JR Yates (2002)  Shotgun identification of protein modifications from protein complexes and lens tissue.  Proc Natl Acad Sci  99:7900-7905. PMCID: PMC122992. (PDF)

38. QYan, JI Clark, TN Wight and EH Sage (2002) Alterations in the lens capsule contribute to cataractogenesis in SPARC-null mice.  J. Cell Sci 115:2747-56. (PDF)

39. JI Clark (2002) sHsps: the neglected chaperones: Progress in Molecular & Subcellular Biol.J Cell Sci 115:2255-2257.

40. S Hu, Y Mingliang, G Surh*, JI Clark & N Dovichi (2002) Analysis of Proteins by Capillary SDS-DALT Electrophoresis with Laser Induced Fluorescence Detection. LC-GC Technology Europe. March: pp 2-6.

41. MM Valdez*, JI Clark, GJ Wu and PJ Muchowski (2002) Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16.3 and human alphaB-crystallin. Eur. J. Biochem. 269: 1806-1813. (PDF)

2001

42. JI Clark (2001) Therapeutic applications of heat shock proteins and molecular chaperones. Expert Opin. Ther. Patents. 11:1053-1062.

43. JI Clark (2001) Fourier and power law analysis of structural complexity in cornea and lens. Micron. 32: 239-249. (PDF)

44. AF Clark, HT Steely, JE Dickerson Jr, S.English-Wright, K Stropki, MD McCartney, N Jacobson,  AR Shepard,  JI Clark,  H Matsushima, ER Peskind, JB Leverenz, CW Wilkinson, RE Swiderski, JH Fingert, VC Sheffield, & EM Stone (2001) Glucocorticoid Induction of the Glaucoma Gene MYOC in Human and Monkey Trabecular Meshwork Cells & Tissues.  Invest. Ophthalmol. Vis. Sci. 42: 1769-1780. (PDF)

45. BK Derham, MA van Boekel, PJ Muchowski, JI Clark, J Horwitz, HW Hepburne-Scott, WW de Jong, MJ Crabbe and JJ Harding (2001) Chaperone function of mutant versions of alpha A- and alpha B-crystallin prepared to pinpoint chaperone binding sites.  Eur J Biochem. 268:713-721. (PDF)

46. SJ Tumminia, JI Clark, DM Richiert, KP Mitton, Y Duglas-Tabor, JA Kowalak, DL Garland and P Russell (2001) Three distinct stages of lens opacification in transgenic mice expressing the HIV-1 protease.  Exp. Eye Res. 72:115-121. (PDF)

2000

47. K Norose, WK Lo, JI Clark, EH Sage and CC Howe (2000) Lenses of SPARC-null mice exhibit an abnormal cell surface-basement membrane interface.  Exp. Eye Res. 71:295-307. (PDF)

48. TR Klesert*, DH Cho*, JI Clark, J Maylie, J Adelman, L Snider, EC Yuen, P Soriano, and SJ Tapscott. (2000) Mice deficient in Six5 develop cataracts: implications for myotonic dystrophy. Nature Genetics. 25:105-109. (PDF)

49. Q Yan*, JI Clark and EH Sage (2000) Expression and characterization of SPARC in human lens and in the aqueous and vitreous humors. Exp. Eye Res. 71:81-90. (PDF)

50. H Matsushima, K Mukai, Y Obara and JI Clark (2000) Analysis of cytoskeletal proteins in rat selenium cataract using 2-dimensional electrophoresis.  Nippon Ganka Gakkai Zasshi. 104(5):299-305.

51. P Parsons-Wingerter, KE Elliott*, JI Clark, and AG Farr. (2000) FGF-2 selectively stimulates the angiogenesis of small vessels in the arterial tree.  Arteriosis, Thrombosis and Vascular Biology. 20:1250-1256. (PDF)

52. P Parsons-Wingerter, KE Elliott*, AG Farr, K Radhakrishnan, JI Clark, and EH Sage. (2000) Generational analysis reveals that TGF-β1 inhibits the rate of angiogenesis in vivo by selective decrease in the number of new vessels. Microvasc Res.59:221-32. (PDF)

53. H Matsushima, K Mukai, Y Obara, H Cai, Y Ito & J.I. Clark (2000) Analysis of proteins during recovery from lens opacity--analysis of selenite cataract model using Sprague-Dawley and Wistar rat.. J Japanese Ophthalmological Soc 104:377-383.

54. JI Clark & PJ Muchowski. (2000) sHSP and their potential in human disease. Curr Opinions in Struct. Biol . 10:52-59. (PDF)

55. H Matsushima, K Mukai, Y Obara and JI Clark (2000) Analysis of cytoskeletal proteins in rat selenium cataract using 2-D electrophoresis. J. Japanese Ophthalmological Soc. 104:299-305.

56. JI Clark & JM Clark. (2000) Lens cytoplasmic phase separation. Int. Rev. Cytol. 192:171-87. JR Kuszak, JI Clark and JM Rae. (1999) Lens transparency, embryology, anatomy, & physiology, in Principles & Practice of Ophthalmology, DM Albert & FA Jakobiec Eds WB.Saunders Co., Philadelphia, PA pp.1305-1409.

57. MD Perng, PJ Muchowski*, P van Den IJssel, GJ Wu, AM Hutcheson, JI Clark &RA Quinlan. (1999) The cardiomyopathy & lens cataract mutation in αB-crystallin alters its protein structure, chaperone activity & interaction with intermediate filaments in vitro. J Biol Chem 274:33235-33243. (PDF)

58. PJ Muchowski*, LG Hays, JR Yates III & J.I. Clark. (1999) ATP and the core "α-crystallin" domain of the small heat shock protein αB-crystallin. J Biol Chem. 274:30190-30195. (PDF)

59. JI Clark, H Matsushima, LL David & JM Clark(1999) Lens cytoskeleton and transparency: a model. Eye 13:417-424.

60. GB Benedek, J Pande, GM Thurston & JI Clark (1999) Theoretical and experimental basis for the inhibition of cataract. Prog. Retin. Eye Res. 18: 391-402. (PDF)

61. PJ Muchowski* MA Valdez* & JI Clark. (1999) B-crystallin selectively targets intermediate filament proteins during thermal stress. Invest. Ophthalmol. Vis. Sci. 40: 951-958. (PDF)

62. JA Bassuk, T Birkebak, JD Rothmier, JM Clark, A Bradshaw, PJ Muchowski, CC Howe, JI Clark & E.H.Sage . (1999) Disruption of the Sparc locus in mice alters the differentiation of the lenticular epithelium and leads to cataract formation. Exp. Eye Res. 68: 321-331. (PDF)

63. PJ Muchowski*, GS Wu*, JN Liang, ET Adman & JI Clark (1999) Site-directed mutations within the core "-crystallin"domain of the sHsp, human B–crystallin decrease molecular chaperone function. J.Mol. Biol.289:397-411. (PDF)

64. JI Clark & T Hiraoka (1999) "Evaluation of anticataractogenic reagents", in:  Nutritional & Environmental Influences on the Eye, A Taylor, Ed. CRC Press, Boca Raton, FL. pp. 117-134.

65. P Parsons-Wingerter, B Lwai, MP Yang, KE Elliott, A Milaninia, A Redlitz, JIClark & EH Sage. (1998) A novel assay of angiogenesis in the quail chorioallantoic membrane: Stimulation by bFGF and inhibition by angiostatin according to fractal dimension and grid intersection. Microvascular Research 55: 201-214.(PDF)

66. PJ Muchowski* & JI Clark. (1998) ATP enhanced molecular chaperone functions of the small heat shock protein B crystallin. Proc. Natl. Acad. Sci. USA 95: 1004-1009. PMCID: PMC18652. (PDF)

67. K Norose, JI Clark, NA Syed, A Basu,  E Heber-Katz, EH Sage. & CC  Howe. (1998) sparc deficiency leads to early-onset cataractogenesis. Invest Ophthalmol Vis Sci 39: 2674-2680. (PDF)

68. H Matsushima*, ES Peskind, JM Clark, JB Leverenz, CW Wilkinson & JI Clark (1997) Protein changes during aging and the effects of long term cortisol treatment on macaque monkey lens. Optometry & Vis Sci 74:190-199. (PDF)

69. PJ Muchowski*, JA Bassuk, NH Lubsen & JI Clark. (1997) Human alpha B crystallin: Small heat shock protein and molecular chaperone. J. Biol. Chem. 272:2578-2582.

70. H Matsushima*, LL David, T Hiraoka & JI Clark (1997) Loss of cytoskeletal proteins and lens cell opacification in the selenite cataract model. Exp. Eye Res. 64: 387-395.

71. GM Thurston, DL Hayden, P Burrows, JI Clark, VG Taret, J Kandel, M Courogen, JA Peetermans, MS Bowen, D Miller, KM Sullivan, R Storb, H Stern and GB Benedek. (1997) Evidence for increased interprotein association as a function of age in the living human lens using quasielastic light scattering. Curr Eye Res. 16: 197-207.

72. JI Clark. (1997) Introduction of George B. Benedek: 1997 Proctor Medal. Invest Ophthalmol Vis Sci  38: 1909-1910. (PDF)

73. JI Clark, and QL Huang. (1996) Modulation of chaperone-like activity of bovine alpha crystallin. Proc. Natl. Acad. Sci. USA. 93: 15185-15189. PMCID: PMC26378. (PDF)

74. J.I. Clark, J.C.Livesey, and J.E. Steele (1996) Delay or inhibition of rat lens opacification using pantethine and WR-77913. Exp. Eye Res. 62: 75-85.

75. T Hiraoka*, JI Clark, XY Li & GM Thurston (1996) Effect of selected anti-cataract agents on opacification in the selenite cataract model. Exp. Eye Res. 62: 11-21.

76. T Hiraoka* & JI Clark (1995) Inhibition of opacification during early stages of cataract formation. Invest . Ophthalmol. Vis. Sci. 36: 2550-2555. (PDF)

77. S Vaezy*, LT Smith, A Milaninia & JI Clark (1995) 2-D Fourier analysis of electron micrographs of human skin for quantification of the collagen fiber organization in the dermis. J. Electron Microsc. 44: 358-364.

78. S Vaezy* & JI Clark (1995) Characterization of the cellular microstructure of ocular lens using 2-D power-law analysis. Ann. Biomed. Eng. 23: 482-490. (Published erratum appears in Ann Biomed. Eng. 23:707-709)

79. S Vaezy*, JI Clark & JM Clark (1995) Quantitative analysis of lens cell microstructure in selenite cataract using a two-dimensional Fourier analysis. Exp. Eye Res. 60: 245-255. (PDF)

80. S Vaezy* & JI Clark (1994) Quantitative analysis of the microstructure of the human cornea and sclera using 2-D Fourier methods. J of Microscopy 175: 93-99.

81. JI Clark (1994) Lens cytoplasmic protein solutions: Analysis of a biologically occurring aqueous phase separation, in "Aqueous Two-Phase Systems," eds. H Walter & G Johansen. Methods in Enzymology 228:525-537.

82. JI Clark (1994) Development and maintenance of lens transparency in "Principles and Practice of Ophthalmology," Ed. DM Albert & FA Jakobiec, WB Saunders Co. Philadelphia, PA, pp. 114-123.

83. JI Clark, JC Livesey and JE Steele (1993) Phase separation inhibitors and lens transparency. Optometry and Vision Sci. 70: 873-879. (PDF)

84. JI Clark & JE Steele (1992) Phase separation inhibitors and prevention of selenite cataract. Proc Natl Acad Sci USA 89:1720-1724. PMCID: PMC48524. (PDF)

85. B. Brewitt*, D. Teller and J.I. Clark (1992) Periods of oscillatory growth in developing ocular lens correspond with cell cycle times. J Cell Physiol 150:586-592.

86. M Gisselberg*, JI Clark, S Vaezy* & TB Osgood* (1991) A quantitative evaluation of Fourier components in transparent and opaque calf cornea.  Amer. J. of Anatomy 191: 408-418.

87. S Vaezy* & JI Clark (1991) A quantitative analysis of transparency in the human sclera and cornea using Fourier methods. J. Microscopy 163:85-94.

88. S Vaezy*, JI Clark & JM Clark (1990) Fourier analysis of two structural organizations in opaque mouse lenses.  Proc. Intl. Congress for Electron Microscopy XII:554-559.

89. JI Clark (1990) Phase Separation and H-Bonding in Cells of the Ocular Lens.  Biopolymers 30:995-999.

90. B Brewitt* & JI Clark (1990) A new method for studying normal lens development in vitro using pulsatile delivery of PDGF or EGF in HL-1 serum free medium. In Vitro Cell. Dev. Biol. 26:305-314. (PDF)

91. CF Beaulieu* & JI Clark (1990) 31P NMR and LASER spectroscopic analyses of lens transparency during calcium induced opacification.  Invest. Ophthal. Vis. Sci. 31(7):1339-1347. (PDF)

92. S Vaezy*, JI Clark, M Gisselberg* & ME Cantino (1989) Fourier analysis of digital STEM images of rat lens in the study of transparency.  Proceedings of IEEE Conference on Engineering in Medicine and Biology 11:369-375.

93. JI Clark & CF Beaulieu* (1989) From EM microprobe analysis to NMRD studies of the lens. Lens & Eye Toxicity Research 6(4): 523-539. (PDF)

94. VN Reddy, H Ikebe, FJ Giblin, JI.Clark & JC Livesey (1989) Effect of Radioprotective Agents on X-ray cataracts.  Lens and Eye Toxicity Res  6(4): 573-588.

95. CF Beaulieu,* RD Brown, JI Clark, M Spiller, & SH Koenig (1989) Relaxometry of lens homogenates II:  temperature dependence and comparison with other proteins.  Magnetic Resonance in Medicine 10:362-372.

96. B Brewitt* & JI Clark (1988) Growth and transparency in the lens, an epithelial tissue, stimulated by pulses of PDGF.  Science. 242: 777-779. (PDF)

97. CF Beaulieu*, JI Clark, RD Brown III, M Spiller & SH Koenig (1988) NMR relaxometry of calf lens homogenates, including cross relaxation by crystallin NH group. Magnetic Resonance in Medicine. 8: 45-57.

98. JI Clark, ME Danford-Kaplan* &M. Delaye (1988) Calcium decreases transparency of homogenate from lens cortex and has no effect on nucleus.  Exp. Eye Res. 47: 447-455. (PDF)

99. CR Kissinger*, S Turley & JI Clark (1988) Structure of WF-3681, 3-(2,5-dihydro-4-hydroxy-5-oxo-3 -phenyl-2-furyl)propionic acid.  Acta Cryst. C44: 512-514.

100. JI Clark, TB Osgood* & SJ Trask* (1987) Inhibition of phase separation by reagents that prevent Xirradiation cataract  in vivo.  Exp. Eye Res. 45: 961-967. (PDF)

101. S Eccarius* & JI Clark (1987) Effect of aspirin and vitamin E on phase separation in calf lens homogenate.  Ophthalmic Res. 19: 65-71.

102. M Delaye, ME DanfordKaplan*, JI Clark, B Krop, T GulikKrzywicki & A Tardieu (1987) Effect of calcium on lens cytoplasm.  Exp. Eye Res. 44: 601-616. (PDF)

103. JI Clark & DL Carper (1987) Phase separation in lens cytoplasm is genetically linked to cataract formation in the Philly mouse.  Proc. Natl. Acad. Sci. USA 84: 122125. PMCID: PMC304154. (PDF)

104. TW Menard*, TB Osgood*, JI Clark, AM Spence, JE Steele, KA Krohn & JC Livesey  (1986) Radioprotection against cataract formation by WR77913 in gamma irradiated rats. Int J Radiation Oncol Biol Physics 12:14831486.

105. TB Osgood*, TW Menard*, JI Clark & K Krohn (1986) Inhibition of lens opacification in Xirradiated rats treated with WR77913.  Invest Ophthalmol Vis Sci 27:17801784. (PDF)

106. CR Kissinger*, ET Adman, RE Stenkamp & J.I. Clark (1985) Crystal structure of sorbinil.  Acta Cryst. C41: 988990.

107. JI Clark & ME Danford* (1985) Low temperature and acrylamide inhibit lens opacification caused by calcium.  Ophthalmic Res. 17: 246250.

108. JR Neuringer*, JI Clark & GB Benedek (1985) Quantitative microprobe analysis of elements in cortical and nuclear cells of calf lens. Anat Rec 211: 329337.

109. JI Clark (1985) Bedeutung der PhaseUmwandlung fur die Kataraktentwicklung? In: Biochemie des Auges.  O. Hockwin, Ed.  Enke Verlag, Stuttgart, pp. 290299.

110. I Nishio, JN Weiss, T Tanaka, JI Clark, FJ Giblin, VN Reddy & GB Benedek (1984) In vivo observation of lens protein diffusivity in normal and Xirradiated rabbit lenses.  Exp. Eye Res. 39: 6168. (PDF)

111. JI Clark, JR Neuringer* & GB Benedek (1983) Phase separation and lens cell age.  J. Gerontol. 38: 287292.

112. M Delaye, JI Clark & GB Benedek (1982) Identification of scattering elements responsible for lens opacification in cold cataracts.  Biophys J 37:647656. PMCID: PMC1328850. (PDF)

113. JI Clark, FJ Giblin, VN Reddy & GB Benedek (1982) Phase separation in Xirradiated lenses of the rabbit.  Invest Ophthal 22:186190. (PDF)

114. JI Clark, M Delaye, P Hammer & L Mengel (1982) Preparation and characterization of native lens cell cytoplasm.  Curr Eye Res. 1:695704.

115. M Delaye, JI Clark & GB Benedek (1981) Light Scattering studies of cataracts. In: Scattering Techniques Applied to Supramolecular & NonEquilibrium Systems. SH Chen, B Chu & R Nossal, Eds., Plenum Press, NY, pp. 879885.

116. MA Delaye, JI Clark&GB Benedek (1981) Coexistence curves for phase separation in the calf lens cytoplasm.  Biochem Biophys Res Comm 100:908914.

117. JI Clark, M Delaye & GB Benedek (1981) Laser light scattering & cataract. In: Proceedings of the Technical Conference on ElectroOptics &Lasers. Industrial & Scientific Conference Pubs, Chicago, IL, p365370.

118. DA Albertini & JI Clark (1981) Visualization of assembled and disassembled microtubule protein by double label fluorescence microscopy.  Cell Biol. Int. Rep. 5:387397.

119. JI Clark & GB Benedek (1980) The effects of glycols, aldehydes & acrylamide on phase separation & opacification in the calf lens. Invest Ophthal 19: 771776. (PDF)

120. JI Clark & GB Benedek (1980) Phase diagram for cell cytoplasm.  Biochem. Biophys. Res. Comm. 95: 482489. (PDF)

121. JI Clark, L Mengel & GB Benedek (1980)  Scanning electron microscopy of opaque and transparent states in reversible calf lens cataracts.  Ophthal. Res. 12: 1633.

122. JI Clark, L Mengel, A.Bagg & GB Benedek (1980) Cortical opacity, calcium concentration & fiber membrane structure in the calf lens.  Exp Eye Res 31: 399410. (PDF)

123. JI Clark and TN Margulis(1980) Structure of colchicoside: Xray analysis of thiocolchicoside, the sulfur analogue of the glucoside of colchicine. Life Sciences 26: 833837. (PDF)

124. GB Benedek & JI Clark (1980) Light scattering in the lens: phase transition and opacification. In: Developments in Biochemistry, Vol. 9, Red Blood Cell and Lens Metabolism.  SK Srivastava, Ed. pp. 431435.

125. GB Benedek, JI Clark, E Serrallach, C Young, L Mengel, T Sauke, A Bagg, & K Benedek (1979) Light scattering & reversible cataracts in calf and human lenses.  Phil Trans Roy Soc (Lond.) Ser. A 293: 329340.

126. JI Clark & D Garland (1978) Fluorescein colchicine: synthesis purification & biological activity. J Cell Biol. 76: 619627. PMCID: PMC2110006. (PDF)

127. JI Clark & DA Albertini (1976) Filaments, microtubules and colchicine receptors in capped ovarian granulosa cells.  Cold Spring Harbor Symposium on Cell Motility, pp. 323331.

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