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Freeze-fracture preparation of mouse sperm cytoprotected in 20% glycerol and fast frozen prior to carbon/platinum replication in a Balzers
301 freeze-fracture apparatus. Electron micrograph recorded in a Philips 201 microscope, digitized using a UMAX 600dpi flatbed scanner, and colorized with "Adobe Photoshop" by painting and
layering with output to a "Lazergraphics" film recorder. Colorization by Joe Wilmhoff. Mag 25,000 x.
Jim Koehler
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