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 Stenkamp, Ronald E., Ph.D.
Professor
(joint with Biochemistry)
Room K-440a HSB
Phone – 206-685-1721
e-mail – stenkamp@u.washington.edu
Personal webpage: http://faculty.washington.edu/stenkamp/
The focus in my group is on the application of X-ray crystallographic techniques to determine the three
-dimensional structures of biologically interesting macromolecules. Understanding the arrangements of
atoms in molecules is useful for asking questions about their chemistry and function.
We are interested in a range of proteins and collaborate extensively with investigators whose emphasis is
on the chemistry and biology of the molecules. Here's a partial list of structure determinations currently underway in my lab.
Rhodopsin. This G protein-coupled receptor is the photo-active molecule in the visual system. In response
to light, it initiates a signaling cascade that results in signals being sent to the brain. We have solved the
ground-state structure as well as a photo-activated form of the protein. We are also pursuing studies of
rhodopsin in complex with its protein ligands. This work is being done in collaboration with Kris Palczewski at Case-Western Reserve University.
Streptavidin. Biotin binds very strongly to streptavidin, and we want to understand the intermolecular
interactions involved. In collaboration with Pat Stayton (Univ. of Washington) and Terry Lybrand
(Vanderbilt Univ.), we are applying biophysical, computational and crystallographic methods to understand
how the protein recognizes its small molecule ligands
In addition to these two major projects, we are also collaborating with other Univ. of Washington faculty
on molecular structures of importance for their functional studies. We currently have projects underway
with Rachel Klevit (Biochemistry), Bill Parson (Biochemistry), Steve Libby (Laboratory Medicine), Steve
Moseley (Microbiology), Evgeni Sokurenko (Microbiology), Bill Atkins (Medicinal Chemistry), and Rheem Totah (Medicinal Chemistry).
For additional information, please link to my personal Home Page.
Selected Publications
Korotkova, N., Y. Yang, I. Le Trong, E. Cota, B. Demeler, J. Marchant, W.E. Thomas, R.E. Stenkamp, S.L.
Moseley and S. Matthews 2008 Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen
triggers receptor dissociation. Molecular Microbiology, 67(2), 420-434.
Le Trong I. and R.E. Stenkamp 2007 An alternate description of two crystal structures of phospholipase A2
from Bungarus caeruleus. Acta Cryst. D63(4), 548-549.
Lodowski, D.T., D. Salom, I. Le Trong, D.C. Teller, J.A. Ballesteros, K. Palczewski and R.E. Stenkamp 2007
Crystal Packing Analysis of Rhodopsin Crystals. J. Struct. Biol., 158, 455-462.
Salom, D., D.T. Lodowski, R.E. Stenkamp, I. Le Trong, M. Golczak, B. Jastrzebska, T. Harris, J.A.
Ballesteros and K. Palczewski 2006 Crystal structure of a photoactivated deprotonated intermediate of
rhodopsin. Proc. Natl. Acad. Sci., USA, 103, 16123-16128.
Hyre, D.E., I. Le Trong, E.A. Merritt, J.F. Eccleston, N.M. Green, R.E. Stenkamp and P.S. Stayton 2006
Cooperative hydrogen-bond interactions in the streptavidin-biotin system. Protein Science, 15, 459-467.
Le Trong, I., N. Humbert, T.R. Ward and R.E. Stenkamp 2006 Crystallographic analysis of a full-length
streptavidin and its C-terminal polypeptide bound in the biotin binding site. J. Mol. Biol., 356, 738-745.
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